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EGFR and beta1 integrins utilize different signaling pathways to activate Akt.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
2008 (English)In: Experimental Cell Research, ISSN 0014-4827, E-ISSN 1090-2422, Vol. 314, no 2, 309-316 p.Article in journal (Refereed) Published
Abstract [en]

Akt, also called PKB, is a serine/threonine kinase that plays a major role in cell survival. It can be activated by several cellular receptors, including integrins and growth factor receptors, in PI3K-dependent manners. In this study, we analyzed the two current models for Akt activation upon beta1 integrin-mediated adhesion: via focal adhesion kinase and via transactivation of the EGF receptor. Distinct differences in the pathways leading to phosphorylation and activation of Akt from stimulated beta1 integrins and EGF receptor were observed, including opposing sensitivity to the tyrosine kinase inhibitors PP2 and Gefitinib. Using knockout cells and integrin mutant cells, we show that beta1 integrins can induce phosphorylation of Akt at Ser473 and Thr308 and Akt kinase activity independently of the EGF receptor activity, focal adhesion kinase, and the Src family members. In contrast to stimulation with EGF, beta1 integrin-mediated adhesion did not induce Akt tyrosine phosphorylation. Moreover, tyrosine phosphorylation of Akt was found not to be required for its catalytic activity. The results identify a previously unrecognized mechanism by which beta1 integrins activate the PI3K/Akt pathway.

Place, publisher, year, edition, pages
2008. Vol. 314, no 2, 309-316 p.
Keyword [en]
beta 1 integrins, EGFR, Akt, Src family kinases, tyrosine phosphorylation
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-103879DOI: 10.1016/j.yexcr.2007.08.018ISI: 000251891300009PubMedID: 17910952OAI: oai:DiVA.org:uu-103879DiVA: diva2:218904
Available from: 2009-05-25 Created: 2009-05-25 Last updated: 2017-12-13Bibliographically approved

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Johansson, Staffan

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