Proteomic identification of p53-dependent protein phosphorylation
2008 (English)In: Oncogene, ISSN 0950-9232, Vol. 27, no 35, 4854-4859 p.Article in journal (Refereed) Published
The p53 tumor suppressor regulates transcription of target genes. We have previously analysed the p53-dependent proteome and identified novel protein targets. Here we have examined p53-dependent phosphorylation using two-dimensional gel electrophoresis and staining with the fluorescent phosphoprotein dye Pro-Q Diamond. We report that p53 induces phosphorylation of a subset of proteins including Nm23, DJ-1, ANXA1 and PrxII. Our identification of p53-dependent phosphorylation of specific target proteins reveals new aspects of the p53-dependent cellular response and suggests that such posttranslational modifications may contribute to p53-mediated tumor suppression.
Place, publisher, year, edition, pages
2008. Vol. 27, no 35, 4854-4859 p.
p53, proteomics, phosphorylation
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-103891DOI: 10.1038/onc.2008.124ISI: 000258445100010OAI: oai:DiVA.org:uu-103891DiVA: diva2:218945