uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Toward a confocal subcellular atlas of the human proteome
Show others and affiliations
2008 (English)In: Molecular & Cellular Proteomics, ISSN 1535-9476, Vol. 7, no 3, 499-508 p.Article in journal (Refereed) Published
Abstract [en]

Information on protein localization on the subcellular level is important to map and characterize the proteome and to better understand cellular functions of proteins. Here we report on a pilot study of 466 proteins in three human cell lines aimed to allow large scale confocal microscopy analysis using protein-specific antibodies. Approximately 3000 high resolution images were generated, and more than 80% of the analyzed proteins could be classified in one or multiple subcellular compartment(s). The localizations of the proteins showed, in many cases, good agreement with the Gene Ontology localization prediction model. This is the first large scale antibody-based study to localize proteins into subcellular compartments using antibodies and confocal microscopy. The results suggest that this approach might be a valuable tool in conjunction with predictive models for protein localization.

Place, publisher, year, edition, pages
2008. Vol. 7, no 3, 499-508 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-103926DOI: 10.1074/mcp.M700325-MCP200ISI: 000254076400004PubMedID: 18029348OAI: oai:DiVA.org:uu-103926DiVA: diva2:219037
Available from: 2009-05-26 Created: 2009-05-26 Last updated: 2009-11-16Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Asplund, AnnaPontén, Fredrik
By organisation
Department of Genetics and Pathology
In the same journal
Molecular & Cellular Proteomics
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 229 hits
ReferencesLink to record
Permanent link

Direct link