Glucolipotoxicity in INS-1E cells is counteracted by carnitine palmitoyltransferase 1 over-expression
2008 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 375, no 4, 517-521 p.Article in journal (Refereed) Published
Effects of non-esterified fatty acids (FAs) are accentuated when applied together with elevated glucose through preferential use of glucose as fuel, which leads to decreased oxidation of FAs. We examined how over-expression of the mitochondrial FA transporter carnitine palmitoyltransferase 1 (CPT1) affects glucose-stimulated insulin secretion (GSIS), apoptosis and ER stress in INS-1E cells cultured in the presence of elevated levels of glucose and palmitate. INS-1E cells were infected with Tet-ON regulated adenovirus containing CPT1 and cultured for 48h in the presence of 0.5mM palmitate and 20mM glucose. Over-expressing CPT1 lowered basal insulin secretion in a dose-dependent manner thereby improving GSIS from INS-1E cells. Also, apoptosis was alleviated and ER-stress markers p-eIF2alpha and CHOP were decreased in cells over-expressing CPT1. We conclude that regulated over-expression of CPT1 is beneficial for glucolipotoxic beta-cells.
Place, publisher, year, edition, pages
2008. Vol. 375, no 4, 517-521 p.
Type 2 diabetes, Glucolipotoxicity, INS-1E, Palmitate, Carnitine palmitoyltransferase 1, ER-stress, GSIS, Apoptosis, p-eIF2 alpha, CHOP/GADD153
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-100420DOI: 10.1016/j.bbrc.2008.08.013ISI: 000259769200006PubMedID: 18706397OAI: oai:DiVA.org:uu-100420DiVA: diva2:219113