Rate and accuracy of bacterial protein synthesis revisited
2008 (English)In: Current Opinion in Microbiology, ISSN 1369-5274, E-ISSN 1879-0364, Vol. 11, no 2, 141-147 p.Article, review/survey (Refereed) Published
Our understanding of the accuracy of tRNA selection on the messenger RNA programmed ribosome has recently increased dramatically because of high-resolution crystal structures of the ribosome, cryo-electron microscopy reconstructions of its functional complexes, and fast kinetics experiments. Application of single-molecule spectroscopy with fluorescence resonance energy transfer to studies of tRNA selection by the ribosome has also provided new, albeit controversial, insights. Interestingly, when the fundamental trade-off between rate and accuracy in substrate-selective biosynthetic reactions is taken into account, some aspects of the current models of ribosome function appear strikingly suboptimal in the context of growing bacterial cells.
Place, publisher, year, edition, pages
2008. Vol. 11, no 2, 141-147 p.
Research subject Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-104032DOI: 10.1016/j.mib.2008.02.015ISI: 000255806600011PubMedID: 18400551OAI: oai:DiVA.org:uu-104032DiVA: diva2:219266