Acute effects of Helicobacter pylori extracts on gastric mucosal blood flow in the mouse
2009 (English)In: World Journal of Gastroenterology, ISSN 1007-9327, E-ISSN 2219-2840, Vol. 15, no 2, 219-225 p.Article in journal (Refereed) Published
AIM: To investigate the mechanisms underlying the reduction in gastric blood flow induced by a luminal water extract of Helicobacter pylori (HPE).
METHODS: The stomachs of isoflurane-anesthetized mice were exteriorized, and the mucosal surface exposed. Blood flow was measured with the laser-Doppler technique, and systemic arterial blood pressure monitored. C57BL/6 mice were exposed to water extract produced from H pylori strain 88-23. To investigate the role of a nerve- or iNOS-mediated pathway, we used intraluminal lidocaine and iNOS-/- mice. Blood flow response to the endogenous nitric oxide synthase inhibitor asymmetric dimethyl arginine (ADMA) was also assessed.
RESULTS: In wild-type mice, HPE decreased mucosal blood flow by approximately 30%. This reduction was abolished in iNOS-deficient mice, and by pre-treatment with lidocaine. Luminally applied ADMA resulted in reduction in blood flow similar to that observed in wild-type mice exposed to HPE.
CONCLUSION: A H pylori water extract reduces gastric mucosal blood flow acutely through iNOS- and nerve-mediated pathways.
Place, publisher, year, edition, pages
2009. Vol. 15, no 2, 219-225 p.
Gastric mucosal blood flow, In vivo, Laser-Doppler flowmetry, Mice, Helicobacter pylori
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-104362DOI: 10.3748/wjg.15.219ISI: 000262739400014PubMedID: 19132773OAI: oai:DiVA.org:uu-104362DiVA: diva2:219690