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The Human Eosinophil Proteome. Changes Induced by Birch Pollen Allergy
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Chemistry. (Inflammation)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
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2009 (English)In: Journal of Proteome Research, ISSN 1535-3893, E-ISSN 1535-3907, Vol. 8, no 6, 2720-2732 p.Article in journal (Refereed) Published
Abstract [en]

Proteins from human eosinophils were separated bidimensionally and identified by mass spectrometry (336 spots/bands, 98 different proteins). Of these, 24.7% belonged to the cytoskeleton/migration group. Highly basic proteins (11.3%) were concentrated in the granule-containing cell fraction. We detected novel hyperacidic forms of cofilin-1, profilin-1 and adenylyl cyclase-associated protein, and hyperbasic forms of eosinophil-derived neurotoxin/eosinophil protein X and major basic protein homologue. We also found evidence of the triglycosylation of the heavy chain of eosinophil peroxidase. In addition, through comparative 2D image analysis, spot quantification and MS, it was found that hsc70, actin-capping protein and hyperacidic forms of eosinophil peroxidase heavy chain are overexpressed in cells from birch pollen allergic subjects, at the peak of a season. The link between these findings and an increased cellular antigen-presenting capacity and motility are discussed.

Place, publisher, year, edition, pages
2009. Vol. 8, no 6, 2720-2732 p.
Keyword [en]
eosinophil fractions, basic proteins, cytoskeletal proteins, birch pollen allergy, hsc70
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-104477DOI: 10.1021/pr800984eISI: 000266719400011PubMedID: 19348509OAI: oai:DiVA.org:uu-104477DiVA: diva2:219844
Available from: 2009-05-28 Created: 2009-05-28 Last updated: 2010-07-26Bibliographically approved

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