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Measuring the affinity of a radioligand with its receptor using a rotating cell dish with in situ reference area
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Oncology, Radiology and Clinical Immunology, Biomedical Radiation Sciences.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Oncology, Radiology and Clinical Immunology, Biomedical Radiation Sciences.
2006 (English)In: Applied Radiation and Isotopes, ISSN 0969-8043, E-ISSN 1872-9800, Vol. 64, no 1, 32-37 p.Article in journal (Refereed) Published
Abstract [en]

This report describes a semi-automated method for the measurement of affinity of radiolabeled ligands interacting with cell-surface receptors on intact cancer cells. The method saves labor time and reagents compared to common manual measurements. A complete affinity measurement can be performed in one cell dish by using a target cell area and a reference area and repeatedly measure the differential activity (i.e. target activity-reference activity). The affinities obtained for different ligand-receptor interactions agreed with affinities reported in the literature.

Place, publisher, year, edition, pages
2006. Vol. 64, no 1, 32-37 p.
Keyword [en]
Antibodies/metabolism, Binding; Competitive, Cell Line; Tumor, Epidermal Growth Factor/*metabolism, Humans, Iodine Radioisotopes, Radioligand Assay/*instrumentation/methods, Receptor; Epidermal Growth Factor/*metabolism, Receptor; erbB-2/*metabolism
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Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-104524DOI: 10.1016/j.apradiso.2005.06.007PubMedID: 16055339OAI: oai:DiVA.org:uu-104524DiVA: diva2:219902
Available from: 2009-05-28 Created: 2009-05-28 Last updated: 2017-12-13Bibliographically approved

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