Measuring the affinity of a radioligand with its receptor using a rotating cell dish with in situ reference area
2006 (English)In: Applied Radiation and Isotopes, ISSN 0969-8043, E-ISSN 1872-9800, Vol. 64, no 1, 32-37 p.Article in journal (Refereed) Published
This report describes a semi-automated method for the measurement of affinity of radiolabeled ligands interacting with cell-surface receptors on intact cancer cells. The method saves labor time and reagents compared to common manual measurements. A complete affinity measurement can be performed in one cell dish by using a target cell area and a reference area and repeatedly measure the differential activity (i.e. target activity-reference activity). The affinities obtained for different ligand-receptor interactions agreed with affinities reported in the literature.
Place, publisher, year, edition, pages
2006. Vol. 64, no 1, 32-37 p.
Antibodies/metabolism, Binding; Competitive, Cell Line; Tumor, Epidermal Growth Factor/*metabolism, Humans, Iodine Radioisotopes, Radioligand Assay/*instrumentation/methods, Receptor; Epidermal Growth Factor/*metabolism, Receptor; erbB-2/*metabolism
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-104524DOI: 10.1016/j.apradiso.2005.06.007PubMedID: 16055339OAI: oai:DiVA.org:uu-104524DiVA: diva2:219902