Automated, high-resolution cellular retention and uptake studies in vitro
2006 (English)In: Applied Radiation and Isotopes, ISSN 0969-8043, E-ISSN 1872-9800, Vol. 64, no 8, 901-905 p.Article in journal (Refereed) Published
This report describes an automated method for the measurements of cellular retention and uptake of radiolabeled proteins interacting with cell-surface receptors on intact cancer cells. A complete uptake and retention measurement was performed in one cell dish using a rotating radioimmunoassay (RIA) principle. Compared to common manual measurements, rotating RIA saved both labor time and reagents and provided real-time binding traces with superior time-resolution. The rotating RIA retention profiles for different interactions agreed with retention times reported in the literature.
Place, publisher, year, edition, pages
2006. Vol. 64, no 8, 901-905 p.
Cellular uptake, Cellular retention, Protein interactions, Kinetics
Radiology, Nuclear Medicine and Medical Imaging
IdentifiersURN: urn:nbn:se:uu:diva-104522DOI: 10.1016/j.apradiso.2006.03.002ISI: 000238809400008PubMedID: 16618544OAI: oai:DiVA.org:uu-104522DiVA: diva2:219903