Correlation between viral resistance to zidovudine and resistance at the reverse transcriptase level for a panel of human immunodeficiency virus type 1 mutants
2001 (English)In: Journal of Virology, ISSN 0022-538X, E-ISSN 1098-5514, Vol. 75, no 15, 7202-7205 p.Article in journal (Refereed) Published
Using a large panel of human immunodeficiency virus type 1 site-directed mutants, we have observed a higher correlation than has previously been demonstrated between zidovudine (AZT)-triphosphate resistance data at the reverse transcriptase (RT) level and corresponding viral AZT resistance. This enhanced-resistance effect at the RT level was seen with ATP and to a lesser extent with PP(i) when ATP was added at physiological concentrations. The ATP-dependent mechanism (analogous to pyrophosphorolysis) appears to be dominant in the mutants bearing the D67N and K70R or 69 insertion mutations, whereas the Q151M mutation seems independent of ATP for decreased binding to AZT-triphosphate.
Place, publisher, year, edition, pages
2001. Vol. 75, no 15, 7202-7205 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-104560DOI: 10.1128/JVI.75.15.7202-7205.2001ISI: 000169870700052PubMedID: 11435603OAI: oai:DiVA.org:uu-104560DiVA: diva2:219945