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Prognostic significance of TP53 accumulation in human primary breast cancer: comparison between a rapid quantitative immunoassay and SSCP analysis.
AB Sangtec Medical.
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1996 (English)In: International Journal of Cancer, ISSN 0020-7136, E-ISSN 1097-0215, Vol. 69, no 2, 125-130 p.Article in journal (Refereed) Published
Abstract [en]

TP53 accumulation in human primary breast carcinomas was studied by a quantitative luminometric immunoassay (LIA), and TP53 gene alterations, exons 5-8, were examined by single-strand conformation polymorphism (SSCP) analysis. In 48 of 142 breast tumor samples, a TP53 gene alteration was identified. In tumor samples without a TP53 gene alteration, the median cytosolic TP53 protein level, as determined by LIA, was 0.4 ng/mg protein (range 0-70.8 ng/mg protein), whereas the median TP53 protein level for tumor samples with a TP53 gene alteration was 10 times higher, i.e., 4.1 ng/mg protein (range 0.1-176.0 ng/mg protein). Despite a significant correlation between the outcome of LIA and SSCP, a disagreement was found in 22% of cases analyzed. Significant correlations were found between TP53 protein accumulation and low estrogen receptor content, and with a shorter relapse-free as well as overall survival, with a median duration of follow-up of 100 months. Due to its rapid and easy performance on routinely prepared cytosols, the LIA for TP53 protein may be useful in evaluating the prognostic impact of TP53 protein accumulation in human primary breast cancer.

Place, publisher, year, edition, pages
1996. Vol. 69, no 2, 125-130 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-104567DOI: 10.1002/(SICI)1097-0215(19960422)69:2<125::AID-IJC10>3.0.CO;2-8ISI: A1996UH13500010PubMedID: 8608980OAI: oai:DiVA.org:uu-104567DiVA: diva2:219950
Available from: 2009-05-28 Created: 2009-05-28 Last updated: 2013-10-25Bibliographically approved

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