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Ligand surface density is important for efficient capture of immunoglobulin and phosphatidylcholine coated particles by human peripheral dendritic cells
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Endocrine Surgery.
2009 (English)In: Cellular Immunology, ISSN 0008-8749, E-ISSN 1090-2163, Vol. 258, no 2, 123-30 p.Article in journal (Refereed) Published
Abstract [en]

A unique property of dealuminated zeolite particles is the exceptional ability to bind both hydrophilic and hydrophobic biomolecules without any covalent linkages. By adsorbing phospholipids onto the particle surface, capture of particles by human peripheral myeloid dendritic cells (mDCs) was observed. Capture of zeolite particles was only seen when a low density of phosphatidylcholine was present on the particles, indicating a specific recognition of the structural features realised by phosphatidylcholine after adsorption on the particle. Adsorbing IgG on the particles revealed capture by mDCs that was dependent upon the density of the IgG molecules. To obtain a smaller particle exposing a high density of IgG molecules, immune complexes (ICs) were formed and both mDCs and pDCs (peripheral plasmacytoid DCs) captured immune complexes, although the mDCs showed a more efficient capture of ICs. As expected, mDCs captured and internalized ICs, whereas pDCs captured ICs but showed no internalization of ICs.

Place, publisher, year, edition, pages
2009. Vol. 258, no 2, 123-30 p.
Keyword [en]
Dealuminated zeolite, Monocytes, Myeloid dendritic cell, Plasmacytoid dendritic cell, Phospholipids, Fcγ-receptor
National Category
Medical and Health Sciences
Research subject
Cell Research
Identifiers
URN: urn:nbn:se:uu:diva-104656DOI: 10.1016/j.cellimm.2009.04.001PubMedID: 19419712OAI: oai:DiVA.org:uu-104656DiVA: diva2:220029
Available from: 2009-05-29 Created: 2009-05-29 Last updated: 2017-12-13Bibliographically approved

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