Assessment of lipids in skeletal muscle by high-resolution spectroscopic imaging using fat as the internal standard: comparison with water referenced spectroscopy
2008 (English)In: Magnetic Resonance in Medicine, ISSN 0740-3194, E-ISSN 1522-2594, Vol. 59, no 6, 1259-1265 p.Article in journal (Refereed) Published
The main purpose of the study was to compare proton (1H) single-voxel MR spectroscopy (MRS) with high-spatial-resolution spectroscopic imaging (MRSI) to determine the lipid content in human skeletal muscle. Unsuppressed water line was used as a concentration reference in the processing of single-voxel spectra. The spectrum from yellow bone marrow with a 100% fat content and probe with the vegetable oil served as internal and external reference for high-spatial-resolution MRSI, respectively. Very good correlation was found between lipid concentrations measured by water referenced single-voxel MRS and high-spatial-resolution MRSI with yellow bone marrow as the internal standard. Excellent correlation was found between total lipid concentrations estimated by high-spatial-resolution MRSI with vegetable oil as the external fat standard and yellow bone marrow as the internal reference. From comparison of single-voxel MRS and MRSI approaches, it follows that relaxation correction of the reference water and methylene fat line is inevitable in processing the standard single-voxel spectra. The high-resolution MRSI approach is recommended to avoid the problem of relaxation corrections and enables using vegetable oil as the external fat standard.
Place, publisher, year, edition, pages
2008. Vol. 59, no 6, 1259-1265 p.
MR spectroscopy, spectroscopic imaging, muscular lipids, quantitation, external and internal fat reference, relaxation corrections
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-104860DOI: 10.1002/mrm.21601ISI: 000256266400006PubMedID: 18421681OAI: oai:DiVA.org:uu-104860DiVA: diva2:220196