uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Role of anti-lipopolysaccharide factor from the black tiger shrimp, Penaeus monodon, in protection from white spot syndrome virus infection
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
Show others and affiliations
2009 (English)In: Journal of General Virology, ISSN 0022-1317, E-ISSN 1465-2099, Vol. 90, no Part 6, 1491-1498 p.Article in journal (Refereed) Published
Abstract [en]

The anti-lipopolysaccharide factor (ALF) from the black tiger shrimp, Penaeus monodon, has been shown previously to exhibit a broad spectrum of activity against various strains of bacteria and fungi. Herein, the recombinant ALFPm3 (rALFPm3) protein was examined for its role in the defence against white spot syndrome virus (WSSV) infection in haematopoietic (Hpt) cell cultures of the freshwater crayfish, Pacifastacus leniusculus, as well as in live P. monodon shrimps. Incubation of Hpt cell cultures with a mixture of WSSV and rALFPm3 resulted in a dose-dependent decrease in VP28 gene expression levels, compared with those incubated with WSSV alone, with an rALFPm3 IC(50) value lower than 2.5 muM. However, pre-treatment of Hpt cells with 5 muM rALFPm3 showed no induced protection against subsequent WSSV infection, whereas the synthetic crayfish ALF peptide could protect cells at a higher concentration (10 muM). The in vivo role of ALFPm3 was examined by injection of P. monodon with WSSV pre-treated with rALFPm3 protein. The results clearly showed that rALFPm3 was able to reduce WSSV propagation and prolong the survival of shrimps.

Place, publisher, year, edition, pages
2009. Vol. 90, no Part 6, 1491-1498 p.
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:uu:diva-105739DOI: 10.1099/vir.0.009621-0ISI: 000266616200023PubMedID: 19264668OAI: oai:DiVA.org:uu-105739DiVA: diva2:222341
Available from: 2009-06-08 Created: 2009-06-08 Last updated: 2017-12-13

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Authority records BETA

Söderhäll, IreneSöderhäll, Kenneth

Search in DiVA

By author/editor
Söderhäll, IreneSöderhäll, Kenneth
By organisation
Comparative PhysiologyDepartment of Organismal Biology
In the same journal
Journal of General Virology
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 530 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf