Properties of a HeLa cell 3' exonuclease specific for degrading poly(A) tails of mammalian mRNA.
1992 (English)In: Journal of Biological Chemistry, ISSN 0021-9258, Vol. 267, no 25, 18154-18159 p.Article in journal (Refereed) Published
A HeLa cell 3'-exonuclease with properties of a mammalian mRNA poly(A) tail-removing enzyme has been characterized. The exonuclease shows high specificity for the poly(A) tail, and it is single strand-specific and requires a 3'-hydroxyl group for its activity. During degradation 5'-AMP is liberated as a product, and a 3'-OH group is left on the last adenosine residue of the remaining poly(A) tail. The activity is inhibited by 5'-AMP and can be competed by poly(A)-containing mRNA or poly(A). Based on these findings we propose a reaction pathway for poly(A) tail removal catalyzed by the HeLa cell poly(A) tail-specific 3' exonuclease.
Place, publisher, year, edition, pages
1992. Vol. 267, no 25, 18154-18159 p.
IdentifiersURN: urn:nbn:se:uu:diva-106239PubMedID: 1355481OAI: oai:DiVA.org:uu-106239DiVA: diva2:224296