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Properties of a HeLa cell 3' exonuclease specific for degrading poly(A) tails of mammalian mRNA.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology, Medical Genetics.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology, Medical Genetics.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
1992 (English)In: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 267, no 25, 18154-18159 p.Article in journal (Refereed) Published
Abstract [en]

A HeLa cell 3'-exonuclease with properties of a mammalian mRNA poly(A) tail-removing enzyme has been characterized. The exonuclease shows high specificity for the poly(A) tail, and it is single strand-specific and requires a 3'-hydroxyl group for its activity. During degradation 5'-AMP is liberated as a product, and a 3'-OH group is left on the last adenosine residue of the remaining poly(A) tail. The activity is inhibited by 5'-AMP and can be competed by poly(A)-containing mRNA or poly(A). Based on these findings we propose a reaction pathway for poly(A) tail removal catalyzed by the HeLa cell poly(A) tail-specific 3' exonuclease.

Place, publisher, year, edition, pages
1992. Vol. 267, no 25, 18154-18159 p.
Identifiers
URN: urn:nbn:se:uu:diva-106239PubMedID: 1355481OAI: oai:DiVA.org:uu-106239DiVA: diva2:224296
Available from: 2009-06-17 Created: 2009-06-17 Last updated: 2017-12-13Bibliographically approved

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