uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Fishing for ancient DNA
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Evolution, Genomics and Systematics, Evolutionary Biology.
Show others and affiliations
2008 (English)In: Forensic Science International: Genetics, Vol. 2, no 2, 104-107 p.Article in journal (Refereed) Published
Abstract [en]

The major problems concerning ancient DNA studies are related to the amount of extractable DNA and the precautions needed to avoid contamination. From the very first step of the analyses, the DNA extraction, these problems must be confronted. There are several extraction methods available for DNA in ancient tissue; several of them are complicated and time consuming. and none of the methods have reached an acceptance level such that they are routinely used on a widespread basis. Here we investigate the efficiency of two methods. one based on magnetic separation of the targeted molecules. and one based oil silica binding. The efficiency rate of these two on the material studied seems to be identical. The silica binding method has the benefit of relative simplicity, but the magnetic separation technique also hits advantages. For example, it is possible to reuse the extract several times for different loci, and it is possible to concentrate all extracted DNA from one locus into one PCR.

Place, publisher, year, edition, pages
2008. Vol. 2, no 2, 104-107 p.
Keyword [en]
Ancient DNA extraction, Silica binding purification, Magnetic separation
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:uu:diva-106452DOI: 10.1016/j.fsigen.2007.09.004ISI: 000261500300003OAI: oai:DiVA.org:uu-106452DiVA: diva2:224949
Available from: 2009-06-23 Created: 2009-06-23 Last updated: 2016-04-25Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Authority records BETA

Anderung, Cecilia

Search in DiVA

By author/editor
Anderung, Cecilia
By organisation
Evolutionary Biology
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 573 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf