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Beta-methylamino-L-alanine analysis by liquid chromatography tandem mass spectrometry with iTRAQ as the derivative
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Analytical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Analytical Chemistry.
2009 (English)In: European journal of mass spectrometry, ISSN 1469-0667, Vol. 15, no 3, 439-443 p.Article in journal (Refereed) Published
Abstract [en]

Amino acid BMMA is produced by cyanobacteria and has been linked to the development of neurodegenerative diseases. We developed a method for quantitative analysis of BMAA in biological samples and plant extracts. The method is utilizing iTRAQ and LC-MS/MS detection using multiple reaction monitoring mode. The method uses 50 pL of sample and has a   Limit of quantitation of 300 ng mL(-1), within-run run imprecision  below 1%. Using this method we analyzed human serum samples, human cerebrospinal fluid samples and extract of the cycad seed. No BMAA could be detected in the human samples. Content of BMAA in the seed was 50 mg kg(-1).

Place, publisher, year, edition, pages
2009. Vol. 15, no 3, 439-443 p.
Keyword [en]
beta-methylamino-L-alanine, BMAA, amyotrophic lateral sclerosis, iTRAQ, quantitation, LC-MS/MS
National Category
Chemical Sciences
Research subject
Analytical Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-106794DOI: 10.1255/ejms.984ISI: 000266031200008PubMedID: 19395779OAI: oai:DiVA.org:uu-106794DiVA: diva2:226740
Available from: 2009-07-03 Created: 2009-07-03 Last updated: 2011-03-30Bibliographically approved

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Bergquist, Jonas

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