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Radioimmunoassay for autoantibodies against interferon omega; its use in the diagnosis of autoimmune polyendocrine syndrome type I
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences. (Autoimmuna sjukdomar (Kämpe))
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences. (Autoimmuna sjukdomar (Kämpe))
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2008 (English)In: Clinical Immunology, ISSN 1521-6616, E-ISSN 1521-7035, Vol. 129, no 1, 163-9 p.Article in journal (Refereed) Published
Abstract [en]

Patients with the autoimmune polyendocrine syndrome I (APS I) have high titers of neutralizing IgG autoantibodies against type I interferons (IFNs), in particular IFN-omega. Until now, the most specific assay has been the antiviral interferon neutralizing assay (AVINA), which has the drawbacks of requiring a cytolytic virus, being cumbersome and difficult to standardise. We have developed a fast and reliable immunoassay based on radiolabelled IFN-omega for quantifying anti-IFN-omega antibodies. Sera from 48 APS I patients were analysed together with those from 5 control groups. All sera from APS I patients were positive for anti-IFN-omega, while, except one serum, all sera from the controls were negative. This method has the advantage over bioassays that it is readily adapted to high throughput. It provides an alternative, sensitive and specific diagnostic test for APS I, and an ideal screening tool to precede mutational analyses of the AIRE gene in suspected APS I cases.

Place, publisher, year, edition, pages
2008. Vol. 129, no 1, 163-9 p.
Keyword [en]
autoimmune, polyendocrine, syndrome I, interferon omega, radioimmunoassay, autoimmune regulator
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-106885DOI: 10.1016/j.clim.2008.07.002ISI: 000259459200019PubMedID: 18708298OAI: oai:DiVA.org:uu-106885DiVA: diva2:227022
Available from: 2009-07-08 Created: 2009-07-08 Last updated: 2017-12-13Bibliographically approved

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