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Comparative proteome profiling of MCF10A and 184A1 human breast epithelial cells emphasized involvement of CDK4 and cyclin D3 in cell proliferation
Karolinska Biomics Center, Department of Oncology-Pathology, Karolinska Institutet, Karolinska University Hospital, Solna, Stockholm, Sweden.
Karolinska Biomics Center, Department of Oncology-Pathology, Karolinska Institutet, Karolinska University Hospital, Solna, Stockholm, Sweden.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm , Ludwig Institute for Cancer Research.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm , Ludwig Institute for Cancer Research.
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2009 (English)In: Proteomics Clinical Applications, ISSN 1862-8354, Vol. 3, no 1, 68-77 p.Article in journal (Refereed) Published
Abstract [en]

Acquiring high proliferation rate is crucial for carcinogenic transformation of cells. We reporthere proteome profiling of human breast epithelial cells with low (184A1) and high (MCF10A)proliferation rates.We identified 183 proteins in 184A1 and 318 proteins in MCF10A cells. Thesedatasets provide the most comprehensive proteome annotations of 184A1 and MCF10A cells.Proteins were taken for identification from 2-D gels in a systematic and unbiased way. Functionalclustering of the identified proteins showed similarities in distribution of proteins to the samefunctional domains, indicating similarities in proteomes of 184A1 and MCF10A cells. Amongobserved differences in protein expression, we validated correlation of expression of endogenouscyclin-dependent kinase 4 (CDK4), cyclin D3, cdc25B, and p38g with cell proliferation. Furthermore,down-regulation of CDK4 and cyclin D3 with specific siRNA inhibited cell proliferation,which emphasized the role of CDK4 and cyclin D3 in enhancement of cell proliferation rate ofhuman breast epithelial cells.

Place, publisher, year, edition, pages
2009. Vol. 3, no 1, 68-77 p.
Keyword [en]
Breast epithelial cells, CDK4, Cyclin D3, Proliferation, Proteome profiling
National Category
Cancer and Oncology Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-106888DOI: 10.1002/prca.200800045ISI: 000262893600008OAI: oai:DiVA.org:uu-106888DiVA: diva2:227062
Available from: 2009-07-09 Created: 2009-07-09 Last updated: 2010-12-14Bibliographically approved

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Gautier, AudeWoksepp, HannaHellman, Ulf

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