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Use of Surface Plasmon Resonance Coupled with Mass Spectrometry Reveals an Interaction between the Voltage-Gated Sodium Channel Type X alpha-Subunit and Caveolin-1
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
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2008 (English)In: Journal of Proteome Research, ISSN 1535-3893, E-ISSN 1535-3907, Vol. 7, no 12, 5333-5338 p.Article in journal (Refereed) Published
Abstract [en]

The combination of surface plasmon resonance and mass spectrometry is emerging as a sensitive tool for the elucidation of protein-protein interactions. With the use of surface plasmon resonance-mass spectrometry, peptides, and brain extracts, we now report a novel interaction between the voltage-gated sodium channel type X alpha-subunit and caveolin-1, the central protein controlling caveolae formation. Surface plasmon resonance binding analyses show that this interaction involves amino acids 85-103 of voltage-gated sodium channel type X alpha-subunit and amino acids 81-100 of caveolin-1, a known scaffolding domain of caveolin-1. It is anticipated that the surface plasmon resonance-mass spectrometry approach utilized in this study will be important for the elucidation of protein-protein network analysis in native tissues including the brain.

Place, publisher, year, edition, pages
2008. Vol. 7, no 12, 5333-5338 p.
Keyword [en]
surface plasmon resonance, protein-protein interactions, cavealoe, lipid rafts, ion channel
National Category
Pharmaceutical Sciences
URN: urn:nbn:se:uu:diva-106993DOI: 10.1021/pr800498tISI: 000261478700028PubMedID: 19367709OAI: oai:DiVA.org:uu-106993DiVA: diva2:227542
Available from: 2009-07-15 Created: 2009-07-15 Last updated: 2011-02-22Bibliographically approved

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