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Use of FTA card for dry collection, transportation and storage of cervical cell specimen to detect high-risk HPV
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
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2009 (English)In: Journal of Clinical Virology, ISSN 1386-6532, E-ISSN 1873-5967, Vol. 46, no 2, 112-116 p.Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: The FTA elute micro card, which enable the collection, transport, and archiving of DNA could be an attractive alternative to a liquid based collection system for detection of human papillomavirus (HPV). OBJECTIVES: To develop a method based on the FTA elute micro card for dry collection of cervical epithelial cell samples, suitable for subsequent PCR-based HPV testing. STUDY DESIGN: The method was evaluated by a comparison of the DNA collected by cytobrush and the regular FTA elute micro card from 50 cervical cell samples. The method was then used to estimate the DNA amount in 1040 samples applied to the indicating FTA elute micro card. RESULT: The agreement in HPV positivity between the cytobrush and FTA samples (94%) was excellent (kappa=0.88, 95% CI 0.748-1). All the 1040 samples on the indicating FTA card had sufficient amounts of genomic DNA (>10 copies of a single copy gene) to be suitable for HPV typing. In 53 of the 1040 women the day in the menstrual cycle was noted, and the copy number during follicular phase day 9-13 was found to be statistically significantly lower than for the other three stages in the menstrual cycle (day 4-8, 14, >14) and during menopause. CONCLUSION: The indicating FTA elute micro card represents a suitable medium for collection of cervical cell samples, although follow-up studies are needed to verify the detection of low frequency HPV types.

Place, publisher, year, edition, pages
2009. Vol. 46, no 2, 112-116 p.
Keyword [en]
FTA card, Cervical carcinoma, Human papillomavirus, Real-time PCR
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-107332DOI: 10.1016/j.jcv.2009.06.021ISI: 000270628700003PubMedID: 19628427OAI: oai:DiVA.org:uu-107332DiVA: diva2:228810
Available from: 2009-08-06 Created: 2009-08-06 Last updated: 2010-07-02Bibliographically approved

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Gustavsson, IngerWilander, ErikGyllensten, Ulf

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