Quantitative overview of N2 fixation in Nostoc punctiforme ATCC 29133 through cellular enrichments and iTRAQ shotgun proteomics
2009 (English)In: Journal of Proteome Research, ISSN 1535-3893, E-ISSN 1535-3907, Vol. 8, no 1, 187-198 p.Article in journal (Refereed) Published
Nostoc punctiforme ATCC 29133 is a photoautotrophic cyanobacterium with the capacity to fix atmospheric N2. Its ability to mediate this process is similar to that described for Nostoc sp. PCC 7120, where vegetative cells differentiate into heterocysts. Quantitative proteomic investigations at both the filament level and the heterocyst level are presented using isobaric tagging technology (iTRAQ), with 721 proteins at the 95% confidence interval quantified across both studies. Observations from both experiments yielded findings confirmatory of both transcriptional studies, and published Nostoc sp. PCC 7120 iTRAQ data. N. punctiforme exhibits similar metabolic trends, though changes in a number of metabolic pathways are less pronounced than in Nostoc sp. PCC 7120. Results also suggest a number of proteins that may benefit from future investigations. These include ATP dependent Zn-proteases, N-reserve degraders and also redox balance proteins. Complementary proteomic data sets from both organisms present key precursor knowledge that is important for future cyanobacterial biohydrogen research.
Place, publisher, year, edition, pages
USA: ACS Publications , 2009. Vol. 8, no 1, 187-198 p.
iTRAQ, Shotgun proteomics, Nostoc punctiforme ATCC 29133, Nostoc sp PCC 73102, Tandem-MS, Dinitrogen Fixation, Heterocysts
Research subject Biochemistry
IdentifiersURN: urn:nbn:se:uu:diva-108462DOI: 10.1021/pr800285vISI: 000262171100021OAI: oai:DiVA.org:uu-108462DiVA: diva2:235948