Coxsackievirus B3 infection increases the tissue levels of BDE-99 in the mouse liver
(English)Manuscript (preprint) (Other academic)
The flame retardant 2,2’,4,4’,5-penta-BDE (BDE-99) is accumulated in human tissues. The common human infection that is caused by coxsackievirus B3 (CVB3) changes the tissue distribution of BDE-99 in exposed mice. In this study we investigated whether CVB3 infection in mice causes dose-dependent changes in the tissue levels of radiolabelled 14C-BDE-99 and whether the tissue levels are associated with virus replication and gene expression of the pro-inflammatory chemokine monocyte chemoattractant protein-1 (MCP-1). Mice were infected with CVB3 or sham-inoculated on day 0, orally treated either with 200 µg 14C-BDE-99/kg bw or 20 mg 14C-BDE-99/kg bw on day 1 and euthanized on day 3. Virus levels and gene expressions were measured using real-time polymerase chain reaction (RT-PCR) and tissue concentrations of 14C-BDE-99 were measured by liquid scintillation counting. A dose-dependent uptake of BDE-99 was observed in both the liver and serum. The BDE-99 level in the liver was further increased at both doses by the CVB3 infection. In addition, at the higher dose, BDE-99 increased the virus amount in the liver, whereas the infection-induced expression of MCP-1 was greatly decreased at the higher BDE-99 dose. In conclusion, BDE-99 dose-dependently increased the levels in the liver and serum and the levels in the liver were further increased by the infection and associated with an increase in virus levels in this organ. However, the infection-induced change in BDE-99 levels was not noted in serum, a fact that may complicate the risk assessment in subclinical and clinically infected individuals.
PBDE; coxsackievirus B3; tissue distribution; virus levels; MCP-1
Research subject Medical Virology; Toxicology
IdentifiersURN: urn:nbn:se:uu:diva-108847OAI: oai:DiVA.org:uu-108847DiVA: diva2:240974