Purification and characterization of a laccase from the basidiomycete Funalia trogii (Berk.) isolated in Tanzania
2009 (English)In: African Journal of Biochemistry Research, ISSN 1996-0778, Vol. 3, no 5, 250-259 p.Article in journal (Refereed) Published
A lignolytic basidiomycete fungus, Funalia trogii (Berk.), was isolated from decayed wood in coastal Tanzania and cultivated in submerged culture. Initially screened crude enzyme filtrate showed complete rhemazol brilliant blue - R (RBBR) decolorization 2,2’-azino-bis (3-ethylbenzthiazoline)-6-sulfonate and guaiacol oxidation after 7 days of incubation. The fungal filtrate had maximum laccase activity of 593 U/ml after 15 days of incubation. A laccase was purified by anion exchange and size exclusion chromatography to good purity as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and isoelectric focusing (IEF). The isolated main component had a molecular weight of ca 58 kDa as determined by MS and an isoelectric point (pI) of 3.8. The optimal pH and temperature range for the purified laccase were 4.0 - 5.0 and 50 - 70 ºC, respectively, using 2, 6-dimethoxyphenol as a substrate.
Place, publisher, year, edition, pages
2009. Vol. 3, no 5, 250-259 p.
Funalia trogii, laccase, anion exchange chromatography, size exclusion chromatography, electrophoresis, isoelectric focusing
Biochemistry and Molecular Biology
Research subject Biochemistry
IdentifiersURN: urn:nbn:se:uu:diva-108902OAI: oai:DiVA.org:uu-108902DiVA: diva2:241387