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Purification and characterization of a laccase from the basidiomycete Funalia trogii (Berk.) isolated in Tanzania
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Biochemistry and Organic Chemistry, Biochemistry. (Johansson)
(Mtui)
University of dar-es-Salaam.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Biochemistry and Organic Chemistry, Biochemistry. (Johansson)
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2009 (English)In: African Journal of Biochemistry Research, ISSN 1996-0778, Vol. 3, no 5, 250-259 p.Article in journal (Refereed) Published
Abstract [en]

A lignolytic basidiomycete fungus, Funalia trogii (Berk.), was isolated from decayed wood in coastal Tanzania and cultivated in submerged culture. Initially screened crude enzyme filtrate showed complete rhemazol brilliant blue - R (RBBR) decolorization 2,2’-azino-bis (3-ethylbenzthiazoline)-6-sulfonate and guaiacol oxidation after 7 days of incubation. The fungal filtrate had maximum laccase activity of 593 U/ml after 15 days of incubation. A laccase was purified by anion exchange and size exclusion chromatography to good purity as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and isoelectric focusing (IEF). The isolated main component had a molecular weight of ca 58 kDa as determined by MS and an isoelectric point (pI) of 3.8. The optimal pH and temperature range for the purified laccase were 4.0 - 5.0 and 50 - 70 ºC, respectively, using 2, 6-dimethoxyphenol as a substrate.

Place, publisher, year, edition, pages
2009. Vol. 3, no 5, 250-259 p.
Keyword [en]
Funalia trogii, laccase, anion exchange chromatography, size exclusion chromatography, electrophoresis, isoelectric focusing
National Category
Biochemistry and Molecular Biology
Research subject
Biochemistry
Identifiers
URN: urn:nbn:se:uu:diva-108902OAI: oai:DiVA.org:uu-108902DiVA: diva2:241387
Available from: 2009-10-02 Created: 2009-10-02 Last updated: 2010-01-21Bibliographically approved

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Johansson, Gunnar

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