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F2-isoprostane induced prostaglandin formation in the rabbit
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences, Clinical Nutrition and Metabolism. (Oxidativ stress och inflammation)
2006 (English)In: Free radical research, ISSN 1071-5762, Vol. 40, no 3, 273-277 p.Article in journal (Refereed) Published
Abstract [en]

F2-isoprostanes, non-enzymatic free radical mediated products of arachidonic acid, have shown to form during various oxidant stress status and have potent biological effects. This study investigates to what extent 8-iso-PGF (a major F2-isoprostane), a bioactive product of lipid peroxidation can modify endogenous prostaglandin F (PGF) formation since prostaglandins are inflammatory as well as potent vasoregulatory substances that modulate diverse important physiological functions, and also form during acute and chronic inflammation. An immediate appearance and disappearance of 8-iso-PGF was seen in both plasma and urine within a short interval after i.v. administration of 43 μg/kg of 8-iso-PGF to the rabbits. A successive but differential formation of PGF resulted in a rapid and pulsatile increase of plasma 15-keto-dihydro-PGF, a major metabolite of primary PGF. Later, this compound was excreted efficiently as intact compound into the urine during the 3 h of experiment. A 8-fold increase of PGF metabolite in plasma at 10 min and 12-fold increase in the urine at 30–60 after the i.v. administration of 8-iso-PGF was observed which continued throughout the 3 h of experiment. This observation suggests that pharmacologically administered or endogenously produced 8-iso-PGF during oxidant stress induces prostaglandin formation presumbly through the classical cyclooxygenase-catalysed arachidonic acid oxidation which might be inflammatory itself to the cells and exerts further vasoconstrictive effects.

Place, publisher, year, edition, pages
Informa Healthcare , 2006. Vol. 40, no 3, 273-277 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-109048DOI: 10.1080/10715760500511492OAI: oai:DiVA.org:uu-109048DiVA: diva2:246091
Available from: 2009-10-08 Created: 2009-10-08 Last updated: 2010-07-20Bibliographically approved

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