One-step rapid determination and purification of puerarin from Radix puerariae by n-octylamine-modified poly(methacrylate-co-ethylene dimethacrylate) monolith
2008 (English)In: Journal of chromatography. B, ISSN 1570-0232, Vol. 871, no 1, 1-6 p.Article in journal (Refereed) Published
n-Octylamine-modified poly(methacrylate-co-ethylene dimethacrylate) monoliths were prepared for rapid screening, determination and one-step purification of puerarin from Radix puerariae (a crude extract of the root of Pueraria lobata). The modified monolith showed a specific surface area of 17.8 m(2) g(-1) an average pore size of 0.76 mu m and a total porosity of 60.8%. Fast separation of R. puerariae crude extract was achieved within 5 min at a flow velocity of 722 cm h(-1) resulting in a puerarin Purity of 97%, with a recovery of 85%. This demonstrates the potential of n-octylamine-modified poly(methacrylate-co-ethylene dimethacrylate) monolith for the rapid analysis and separation of isoflavonoids. Preparative scale sample loading (12 mg in 2 mL) resulted in a purity of 95%, and a recovery of about 69%. HPLC, FTIR, MS and H-1 NMR spectroscopy were used for the characterization and quantification of puerarin in isolated fraction.
Place, publisher, year, edition, pages
2008. Vol. 871, no 1, 1-6 p.
monolith, chromatography, Radix puerariae, puerarin, isoflavonoids
IdentifiersURN: urn:nbn:se:uu:diva-109123DOI: 10.1016/j.jchromb.2008.06.010ISI: 000258584100001OAI: oai:DiVA.org:uu-109123DiVA: diva2:248922