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Microfluidic high viability neural cell separation using viscoelastically tuned hydrodynamic spreading
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Micro Structural Technology.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Micro Structural Technology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience.
2008 (English)In: Biomedical microdevices (Print), ISSN 1387-2176, E-ISSN 1572-8781, Vol. 10, no 5, 631-638 p.Article in journal (Refereed) Published
Abstract [en]

A high viability microfluidic cell separation technique of high throughput was demonstrated based on size difference continuous mode hydrodynamic spreading with viscoelastic tuning. Using water with fluorescent dye as sample fluid and in parallel introducing as elution a viscoelastic biocompatible polymer solution of alginic sodium, the spreading behavior was investigated at different polymer concentrations and flow rates. Particle separation was studied in the same detail for 9.9 mu m and 1.9 mu m latex beads. Using buffered aqueous solutions and further surface treatments to protect from cell adhesion, separation between neuron cells and glial cells from rat's spine cord was demonstrated and compared to the separation of latex particles of 20 and 4.6 mu m sizes. High relative viability (above 90%) of neural cells was demonstrated compared the reference cells of the same batch.

Place, publisher, year, edition, pages
2008. Vol. 10, no 5, 631-638 p.
Keyword [en]
microfluidics, viscoelastic, neural cell, separation
National Category
Medical and Health Sciences Engineering and Technology
Identifiers
URN: urn:nbn:se:uu:diva-109131DOI: 10.1007/s10544-008-9174-7ISI: 000258581700004OAI: oai:DiVA.org:uu-109131DiVA: diva2:248941
Available from: 2009-10-09 Created: 2009-10-09 Last updated: 2017-12-13Bibliographically approved

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Wu, ZhigangHjort, Klas

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