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Carbon and energy metabolism
Sveriges lantbruksuniversitet, Inst för växtbiologi och skogsgenetik.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
2009 (English)In: The moss Physcomitrella patens / [ed] Celia Knight, Pierre-François Perroud, David Cove, Oxford: Wiley-Blackwell , 2009, 211-245 p.Chapter in book (Other academic)
Abstract [en]

As in all organisms, the primary carbon metabolism in Physcomitrella patens provides the cells with free energy and building blocks for the biosynthesis of macromolecules. In particular, carbohydrates play a major role in growth and development in mosses as in other plants, since they are used for storage and allocation of carbon and energy. Reverse genetics in P. patens has recently made it possible to address some key questions concerning the carbon and energy metabolism in plants. These questions include: How is the cellular energy status sensed in order to balance anabolism versus catabolism? How does carbon and energy allocation between cells and tissues work? How is whole-plant energy homeostasis maintained? What is the role of sugars in regulating growth and development? And, finally, how similar or different are these processes in bryophytes and vascular plants? The recent release of the complete P. patens genome sequence has now made it possible to address the last question using a comparative genomics approach.

Place, publisher, year, edition, pages
Oxford: Wiley-Blackwell , 2009. 211-245 p.
Series
Annual plant reviews, ISSN 1460-1494 ; 36
Keyword [en]
AMP-activated protein kinase, carbon metabolism, energy homeostasis, hexokinase, SNF1, sugar transport
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-109189ISBN: 978-1-4051-8189-1 (print)OAI: oai:DiVA.org:uu-109189DiVA: diva2:272022
Available from: 2009-10-13 Created: 2009-10-11 Last updated: 2011-01-03Bibliographically approved
In thesis
1. Studies of the Carbon and Energy Metabolism in the Moss Physcomitrella patens
Open this publication in new window or tab >>Studies of the Carbon and Energy Metabolism in the Moss Physcomitrella patens
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Since a proper balance between anabolic and catabolic reactions is essential for all eukaryotes, the basic mechanisms for regulation of the energy and carbon metabolism have been conserved throughout evolution. The moss Physcomitrella patens, which belongs to one of the basal clades among land plants, has many unique properties that make it an excellent plant model system.

We have used a yeast two-hybrid system to identify novel possible regulators or targets of the moss Snf1-related kinases, previously shown to regulate energy homeostasis. The function of the identified interactors PpSki1 and PpSki2 was analyzed in order to better understand the biological role of plant Snf1-related kinases.

The recently completed genome sequence of Physcomitrella was used in a comparative approach to study to what extent key enzyme and gene families involved in transport and metabolism of sugars and in regulation of the energy and carbon metabolism are conserved between mosses and vascular plants.

It has long been known that transformed DNA can replicate episomally in Physcomitrella. We have now shown that such DNA can be rescued back into E. coli. Surprisingly, we found that the original plasmid can be recovered from moss transformants obtained with circular DNA. Plasmids rescued from transformants obtained with linearized DNA had been repaired either by homologous recombination or by cohesive end re-ligation. These findings suggest that methods using shuttle plasmids are feasible in Physcomitrella.

Hexokinase, a key enzyme in the carbon metabolism, catalyzes the first step in hexose metabolism, but is also involved in sugar sensing and signaling. We have now made an initial characterization of the complete hexokinase family in Physcomitrella which is encoded by 11 genes. Two new types of plant hexokinases, types C and D, were found in addition to the previously described types A and B.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2009. 73 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 1651-6206 ; 490
Keyword
Hexokinase, SnRK1, sugar signaling, carbon metabolism, Physcomitrella patens, shuttle vector, subcellular localization, model organism
National Category
Cell and Molecular Biology
Research subject
Molecular Biology
Identifiers
urn:nbn:se:uu:diva-109358 (URN)978-91-554-7631-1 (ISBN)
Public defence
2009-11-27, B41, Uppsala Biomedical Center (BMC), Husargatan 3, 75123 Uppsala, 09:15 (English)
Opponent
Supervisors
Available from: 2009-11-05 Created: 2009-10-14 Last updated: 2009-11-05Bibliographically approved

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Nilsson, Anders

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