Rescue and characterization of episomally replicating DNA from the moss Physcomitrella
2009 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 106, no 46, 19444-19449 p.Article in journal (Refereed) Published
The moss Physcomitrella is unique among plants in that it permits efficient gene targeting by homologous recombination. Furthermore, transformed DNA can replicate episomally in Physcomitrella. Here we show that episomally replicating DNA can berescued back into E. coli, and use such rescue to study the fate of the transformed DNA. Significantly, plasmids rescued from moss transformed with circular DNA are identical to the original plasmid, whereas plasmids rescued from moss transformed with linearized DNA frequently have deletions created by direct repeat recombination.These events are highly predictable in that they target the longest direct repeat on the plasmid, if this repeat is at least 12 bp. Episomal transformants obtained with linearized DNA show a more than 1000-fold amplification of the DNA whereas transformants obtained with circular DNA have much lower copy numbers. Most episomal transformants quickly lose the plasmid in the absence of selection, but a semi-stable type of transformant that loses the plasmid at a much lower frequency was also observed. The consistent rescue of the original plasmid, or of predictable derivatives thereof, suggests that molecular genetics methods which rely on shuttle plasmids are feasible in Physcomitrella
Place, publisher, year, edition, pages
2009. Vol. 106, no 46, 19444-19449 p.
Physcomitrella patens, shuttle plasmid, plasmid rescue, recombination
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-109191DOI: 10.1073/pnas.0908037106ISI: 000271907400041PubMedID: 19892729OAI: oai:DiVA.org:uu-109191DiVA: diva2:272023