uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Incorporation of antimicrobial compounds in mesoporous silica film monolith
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.
Show others and affiliations
2009 (English)In: Biomaterials, ISSN 0142-9612, E-ISSN 1878-5905, Vol. 30, no 29, 5729-5736 p.Article in journal (Refereed) Published
Abstract [en]

Incorporation of the antimicrobial peptide LL-37 (LLGDFFRKSKEKIGKEFKRIVQRIKDFLRNLVPRTES), as well as low molecular weight antimicrobial chlorhexidine, into mesoporous silica was obtained using an EISA one-pot synthesis method. FTIR confirmed efficient encapsulation of both LL-37 and chlorhexidine into mesoporous silica, while XRD and TEM showed that antimicrobial agent incorporation can be achieved without greatly affecting the structure of the mesoporous silica. The modified mesoporous silica released LL-37 and chlorhexidine slowly, reaching maximum release after about 200 h. The release rate could also be controlled through incorporation of SH groups in the pore walls, adding to pore hydrophobicity and reducing the release rate by about 50% compared to the unmodified mesoporous silica. Mesoporous silica containing either LL-37 or chlorhexidine displayed potent bactericidal properties against both Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. While chlorhexidine-loaded mesoporous silica displayed an accompanying high toxicity, as judged from hemolysis, LDH release, and MTT assay, the corresponding material containing LL-37 showed very low toxicity by all these assays, comparable to that observed for mesoporous silica in the absence of antibacterial drug, as well as to the negative controls in the respective assays. Mesoporous silica containing LL-37 therefore holds potential as an implantable material or a surface coating for such materials, as it combines potent bactericidal action with low toxicity, important features for controlling implant-related infections, e.g., for multi-resistant pathogens or for cases where access to the infection site of systemically administered antibiotics is limited due to collagen capsule formation or other factors.

Place, publisher, year, edition, pages
2009. Vol. 30, no 29, 5729-5736 p.
Keyword [en]
Antimicrobial, Chlorhexidine, LL-37, Mesoporous, Silica
National Category
Engineering and Technology
URN: urn:nbn:se:uu:diva-109440DOI: 10.1016/j.biomaterials.2009.07.003ISI: 000270115200053PubMedID: 19628277OAI: oai:DiVA.org:uu-109440DiVA: diva2:272458
Available from: 2009-10-15 Created: 2009-10-15 Last updated: 2016-04-14Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Kupferschmidt, Natalia
By organisation
Nanotechnology and Functional MaterialsDepartment of Pharmacy
In the same journal
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 396 hits
ReferencesLink to record
Permanent link

Direct link