Transcriptional changes in Giardia during host-parasite interactions
2011 (English)In: International Journal for Parasitology, ISSN 0020-7519, Vol. 41, no 3-4, 277-285 p.Article in journal (Refereed) Published
Giardia intestinalis is one of the major causes of parasite-induced diarrhea. The disease, giardiasis, is caused by trophozoites attaching to the intestinal epithelium, resulting in apoptosis of intestinal epithelial cells, disrupted epithelial barrier function and malabsorption. Microarray studies have detected gene expression changes in intestinal epithelial cells (IECs), during interaction with Giardia trophozoites in vitro. In the present study we examined this host-parasite interaction further by transcriptional profiling of interacting trophozoites using Giardia oligo microarrays. A total of 325 Giardia transcripts were significantly changed during the interaction, lasting 1.5 to 18 hrs in complete DMEM medium. Quantitative real-time reverse transcriptase PCR confirmed these changes in all 12 genes tested using mRNA isolated in a separate experiment. Genes involved in cell division and attachment were down-regulated in the late time-points of interaction. Proteins earlier suggested to be important during host parasite interactions like arginine deiminase and cysteine proteinases changed their expression. Oxygen defense proteins and several members of the high cysteine-rich membrane protein (HCMp) family were up-regulated during the interaction with IECs. Thus, there are extensive gene-expression changes in Giardia trophozoites and IECs during host-parasite interactions and this can be important for establishment of infection and in the induction of giardiasis.
Place, publisher, year, edition, pages
2011. Vol. 41, no 3-4, 277-285 p.
Protozoa, intestinal epithelial cell, diarrhea, microarray
Biochemistry and Molecular Biology Infectious Medicine
Research subject Microbiology
IdentifiersURN: urn:nbn:se:uu:diva-109887DOI: 10.1016/j.ijpara.2010.09.011ISI: 000288736700002OAI: oai:DiVA.org:uu-109887DiVA: diva2:274443