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Haplotype-specific expression of the human PDGFRA gene correlates with the risk of glioblastomas
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology.
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2008 (English)In: International Journal of Cancer, ISSN 0020-7136, Vol. 123, no 2, 322-329 p.Article in journal (Refereed) Published
Abstract [en]

Aberrant expression of the platelet-derived growth factor a-receptor (PDGFRA) gene has been associated with various diseases, including neural tube defects and gliomas. We have previously identified 5 distinct haplotypes for the PDGFRA promoter region, designated H1, H2 alpha, H2 beta, H2 gamma and H2 delta. Of these haplotypes H1 and H2 alpha: are the most common, whereby H1 drives low and H2 alpha high transcriptional activity in transient transfection assays. Here we have investigated the role of these PDGFRA promoter haplotypes in gliomagenesis at both the genetic and cellular level. In a case-control study on 71 glioblastoma patients, we observed a clear underrepresentation of H1 alleles, with pH1 = 0.141 in patients and pH1 = 0.211 in a combined Western European control group (n = 998, p < ; 0.05). Furthermore, in 3 out of 4 available H1/H2 alpha heterozygous human glioblastoma cell lines, H1-derived mRNA levels were more than 10-fold lower than from H2 alpha, resulting at least in part from haplotype-specific epigenetic differences such as DNA methylation and histone acetylation. Together, these results indicate that PDGFRA promoter haplotypes may predispose to gliomas. We propose a model in which PDGFRA is upregulated in a haplotype-specific manner during neural stem cell differentiation, which affects the pool size of cells that can later undergo gliomagenesis.

Place, publisher, year, edition, pages
2008. Vol. 123, no 2, 322-329 p.
Keyword [en]
PDGFRA promoter haplotype, allele-specific expression, DNA methylation, glioblastoma, case-control study
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-110041DOI: 10.1002/ijc.23432ISI: 000256760300011PubMedID: 18464291OAI: oai:DiVA.org:uu-110041DiVA: diva2:275063
Available from: 2009-11-03 Created: 2009-11-02 Last updated: 2010-01-22Bibliographically approved

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