Subattomole sensitivity in biological accelerator mass spectrometry
2008 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 80, no 10, 3515-3521 p.Article in journal (Refereed) Published
The Uppsala University 5 MV Pelletron tandem accelerator has been used to study C-14-labeled biological samples utilizing accelerator mass spectrometry (ANIS) technology. We have adapted a sample preparation method for small biological samples down to a few tens of micrograms of carbon, involving among others, miniaturizing of the graphitization reactor. Standard AMS requires about 1 mg of carbon with a limit of quantitation of about 10 amol. Results are presented for a range of small sample sizes with concentrations down to below 1 pM of a pharmaceutical substance in human blood. It is shown that C-14-labeled molecular markers can be routinely measured from the femtomole range down to a few hundred zepto-mole (10(-21) mol), without the use of any additional separation methods.
Place, publisher, year, edition, pages
2008. Vol. 80, no 10, 3515-3521 p.
Subatomic Physics Engineering and Technology
Research subject Ion Physics
IdentifiersURN: urn:nbn:se:uu:diva-110208DOI: 10.1021/ac800174jISI: 000255871500001OAI: oai:DiVA.org:uu-110208DiVA: diva2:275546