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Expression and immunogenicity of six putative variable surface proteins in Mycoplasma mycoides subsp mycoides SC
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Evolution, Genomics and Systematics, Molecular Evolution.
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2008 (English)In: Microbiology, ISSN 1350-0872, Vol. 154, no 2, 539-549 p.Article in journal (Refereed) Published
Abstract [en]

Variable surface protein Vmm and five Vmm-type proteins from Mycoplasma mycoides subsp. mycoides SC were analysed to determine whether these proteins are expressed in vivo in animals affected by contagious bovine pleuropneumonia (CBPP) and in vitro. Recombinant versions of these proteins were constructed and expressed in Escherichia coli after mutation of the TGA Trp codons to TGG. These proteins were then analysed by dot and Western blotting with sera from CBPP-affected cattle. Furthermore, affinity-purified polyclonal antibodies to the recombinant proteins were used in Western and colony blotting to look for expression of the putative Vmm-type proteins in cultured M. mycoides SC. This study demonstrates that immunoglobulins in CBPP sera recognize all putative Vmm-type proteins tested, indicating that these proteins or their homologues are expressed by mycoplasmas during natural infections. Vmm and one of the putative Vmm-type proteins showed variable expression in vitro.

Place, publisher, year, edition, pages
2008. Vol. 154, no 2, 539-549 p.
National Category
Biological Sciences
URN: urn:nbn:se:uu:diva-110487DOI: 10.1099/mic.0.2007/010694-0ISI: 000253927800020OAI: oai:DiVA.org:uu-110487DiVA: diva2:277193
Available from: 2009-11-16 Created: 2009-11-16 Last updated: 2010-02-02Bibliographically approved

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