Nanoporosity of alumina surfaces induces different patterns of activation in adhering monocytes/macrophages
2010 (English)In: International Journal of Biomaterials, ISSN 1687-8787, E-ISSN 1687-8795, Vol. 2010, 402715- p.Article in journal (Refereed) Published
The present study shows that alumina nanotopography affects monocyte/macrophage behaviour. Human mononuclear cells cultured on alumina membranes with pore diameters of 20 and 200 nm were evaluated in terms of cell adhesion, viability, morphology and release of pro-inflammatory cytokines. After 24 hours, cell adhesion was assessed by means of light microscopy and cell viability by measuring LDH release. The inflammatory response was evaluated by quantifying interleukin-1ß and tumour necrosis factor-α. Finally, scanning electron microscopy was used to study cell morphology. Results showed pronounced differences in cell number, morphology and cytokine release depending on the nanoporosity. Few but highly activated cells were found on the 200 nm porous alumina, while relatively larger number of cells was found on the 20 nm porous surface. However, despite their larger number, the cells adhering on the 20 nm surface exhibited reduced pro-inflammatory activity. It can be speculated that the difference in surface topography may lead to distinct protein adsorption patterns and therefore to different degree of cell activation. The data of this paper emphasize the role played by the material nanotexture in dictating cell responses and implies that nanotopography could be exploited for controlling the inflammatory response to implants.
Place, publisher, year, edition, pages
2010. Vol. 2010, 402715- p.
macrophages, nanoporous alumina, biomaterials, nanotopography, inflammatory response
Other Basic Medicine
Research subject Immunology
IdentifiersURN: urn:nbn:se:uu:diva-110623DOI: 10.1155/2010/402715PubMedID: 21234322OAI: oai:DiVA.org:uu-110623DiVA: diva2:277468