Crystallization of Mycobacterium smegmatis methionyl-tRNA synthetase in the presence of methionine and adenosine
2009 (English)In: Acta Crystallographica. Section F: Structural Biology and Crystallization Communications, ISSN 1744-3091, E-ISSN 1744-3091, Vol. 65, no Part 6, 618-620 p.Article in journal (Refereed) Published
Methionyl-tRNA synthetase (MetRS) from Mycobacterium smegmatis was recombinantly expressed in Escherichia coli and purified using Ni(2+)-affinity and size-exclusion chromatography. Crystals formed readily in the presence of the ligands methionine and adenosine. These two ligands are components of an intermediate in the two-step catalytic mechanism of MetRS. The crystals were produced using the vapour-diffusion method and a full data set to 2.1 A resolution was collected from a single crystal. The crystal belonged to the monoclinic space group C2, with unit-cell parameters a = 155.9, b = 138.9, c = 123.3 A, beta = 124.8 degrees . The presence of three molecules in the asymmetric unit corresponded to a solvent content of 60% and a Matthews coefficient of 3.1 A(3) Da(-1). Structure determination is in progress.
Place, publisher, year, edition, pages
2009. Vol. 65, no Part 6, 618-620 p.
methionyl-tRNA synthetase, MetRS, Mycobacterium tuberculosis, methionine, adenosine
IdentifiersURN: urn:nbn:se:uu:diva-110780DOI: 10.1107/S1744309109016704ISI: 000266452100020PubMedID: 19478446OAI: oai:DiVA.org:uu-110780DiVA: diva2:278275