uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Rapid lab-on-a-chip profiling of human gut bacteria
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Micro Structural Technology.
Show others and affiliations
2008 (English)In: Journal of Microbiological Methods, ISSN 0167-7012, E-ISSN 1872-8359, Vol. 72, no 1, 82-90 p.Article in journal (Refereed) Published
Abstract [en]

The human gut microbiota has a substantial impact on human health. Different factors such as disease, diet and drug use can have significant impacts on the gut microbiota. Therefore, it is of interest to have simple, rapid methods for analysis of the composition of the gut microbiota for clinical diagnostic purposes. Since only a minor fraction of the gastrointestinal bacterial community is presently possible to cultivate, molecular approaches are currently the best suited to investigate its composition. However, most of these molecular approaches require technical expertise and expensive equipment to run and they are not routinely available. Ideally, the analyses should be point-of-care options that can be run on a chip. In this study, an existing lab-on-chip (LOC) system for sizing/quantifying DNA was combined with length heterogeneity PCR (LH-PCR), a PCR-based profiling method targeting bacterial 16S rRNA gene sequences, to develop a fast, straightforward, reproducible, and economical method for profiling bacterial communities. The LOC LH-PCR method was first evaluated using a standardized gut cocktail containing genomic DNA from eight different bacterial species representing different genera of relevance for human health. The method was also tested on DNA that was directly extracted from human faecal samples and it was consistently capable of detecting alterations in the bacterial samples before and after antibiotic treatment. Although the resolution of the method needs improvement, this study represents the first step towards development of a diagnostic LOC for profiling gut bacterial communities.

Place, publisher, year, edition, pages
2008. Vol. 72, no 1, 82-90 p.
Keyword [en]
gut cocktail, human gut microbiota, lab-on-a-chip (LOC), length heterogeneity PCR (LH-PCR), microbiome
National Category
Engineering and Technology
URN: urn:nbn:se:uu:diva-111572DOI: 10.1016/j.mimet.2007.10.011ISI: 000252998900011PubMedID: 18055047OAI: oai:DiVA.org:uu-111572DiVA: diva2:281684
Uppsala Microbiomics Center
Available from: 2009-12-17 Created: 2009-12-17 Last updated: 2016-04-12Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Hjort, Klas
By organisation
Micro Structural Technology
In the same journal
Journal of Microbiological Methods
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 392 hits
ReferencesLink to record
Permanent link

Direct link