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Cytochrome P450 1D1: A novel CYP1A-related gene that is not transcriptionally activated by PCB126 or TCDD
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Environmental Toxicology.
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2009 (English)In: Archives of Biochemistry and Biophysics, ISSN 0003-9861, Vol. 482, no 1-2, 7-16 p.Article in journal (Refereed) Published
Abstract [en]

Enzymes in the cytochrome P450 1 family oxidize many common environmental toxicants. We identified a new CYP1, termed CYP1D1, in zebrafish. Phylogenetically, CYP1D1 is paralogous to CYP1A and the two share 45% amino acid identity and similar gene structure. In adult zebrafish, CYP1D1 is most highlyexpressed in liver and is relatively highly expressed in brain. CYP1D1 transcript levels were higher at 9 h post-fertilization than at later developmental times. Treatment of zebrafish with potent aryl hydrocarbon receptor (AHR) agonists (3,3',4,4',5-pentachlorobiphenyl or 2,3,7,8-tetrachlorodibenzo-p-dioxin) did not induce CYP1D1 transcript expression. Morpholino oligonucleotide knockdown of AHR2, which mediates induction of other CYP1s, did not affect CYP1D1 expression. Zebrafish CYP1D1 heterologously expressed in yeast exhibited ethoxyresorufin- and methoxyresorufin-O-dealkylase activities. Antibodies against a CYP1D1 peptide specifically detected a single electrophoretically-resolved protein band in zebrafish liver microsomes, distinct from CYP1A. CYP1D1 in zebrafish is a CYP1A-like gene that could have metabolic functions targeting endogenous compounds.

Place, publisher, year, edition, pages
Amsterdam: Elsevier inc. , 2009. Vol. 482, no 1-2, 7-16 p.
Keyword [en]
zebrafish, CYP1D1, embryo, development, gene expression
National Category
Biochemistry and Molecular Biology
Research subject
URN: urn:nbn:se:uu:diva-111849DOI: 10.1016/j.abb.2008.12.002OAI: oai:DiVA.org:uu-111849DiVA: diva2:283431
Available from: 2010-01-08 Created: 2009-12-27 Last updated: 2010-01-08Bibliographically approved

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