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Neural crest stem cells increase beta cell proliferation and improve islet function in co-transplanted murine pancreatic islets
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Neuroanatomy. (Neuroanatomy)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Neuroanatomy. (Neuroanatomy)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Neuroanatomy. (Neuroanatomy)
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2009 (English)In: Diabetologia, ISSN 0012-186X, E-ISSN 1432-0428, Vol. 52, no 12, 2594-2601 p.Article in journal (Refereed) Published
Abstract [en]

AIMS/HYPOTHESIS: Long-term graft survival after islet transplantation to patientswith type 1 diabetes is insufficient, necessitating the development of newstrategies to enhance transplant viability. Here we investigated whetherco-transplantation of neural crest stem cells (NCSCs) with islets improves islet survival and function in normoglycaemic and diabetic mice. METHODS: Islets alone or together with NCSCs were transplanted under the kidney capsule tonormoglycaemic or alloxan-induced diabetic mice. Grafts were analysed for size,proliferation, apoptosis and insulin release. In diabetic recipients bloodglucose levels were examined before and after graft removal. RESULTS: In mixedtransplants NCSCs actively migrated and extensively associated withco-transplanted pancreatic islets. Proliferation of beta cells was markedlyincreased and transplants displayed improved insulin release in normoglycaemicmice compared with those receiving islet-alone transplants. Mixed grafts survivedsuccessfully and partially restored normoglycaemia in alloxan-induced diabeticmice. CONCLUSIONS/INTERPRETATION: Co-grafting of NCSCs with pancreatic isletsimproved insulin release in mixed transplants and enhanced beta cellproliferation, resulting in increased beta cell mass. This co-transplantationmodel offers an opportunity to restore neural-islet interactions and improveislet functions after transplantation.

Place, publisher, year, edition, pages
2009. Vol. 52, no 12, 2594-2601 p.
Keyword [en]
Diabetes, Islets of Langerhans, Neural stem cells, Transplantation, Trophic factor
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-111981DOI: 10.1007/s00125-009-1544-zISI: 000271723800018OAI: oai:DiVA.org:uu-111981DiVA: diva2:284197
Available from: 2010-01-05 Created: 2010-01-05 Last updated: 2017-12-12Bibliographically approved

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