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Analysis of Genes, Transcripts, and Proteins via DNA Ligation
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology.
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2009 (English)In: Annual review of analytical chemistry, ISSN 1936-1327, Vol. 2, 215-239 p.Article, review/survey (Refereed) Published
Abstract [en]

Analytical reactions in which short DNA strands are used in combination with DNA ligases have proven useful for measuring, decoding, and locating most classes of macromolecules. Given the need to accumulate large amounts of precise molecular information from biological systems in research and in diagnostics, ligation reactions will continue to offer valuable strategies for advanced analytical reactions. Here, we provide a basis for further development of methods by reviewing the history of analytical ligation reactions, discussing the properties of ligation reactions that render them suitable for engineering novel assays, describing a wide range of successful ligase-based assays, and briefly considering future directions.

Place, publisher, year, edition, pages
2009, 2. Vol. 2, 215-239 p.
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Medical and Health Sciences
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URN: urn:nbn:se:uu:diva-112110DOI: 10.1146/annurev-anchem-060908-155239ISI: 000268069300011PubMedID: 20636060OAI: oai:DiVA.org:uu-112110DiVA: diva2:284931
Available from: 2010-01-08 Created: 2010-01-08 Last updated: 2011-01-15Bibliographically approved

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Söderberg, OlaNilsson, MatsLandegren, Ulf

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