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Access channels and methanol binding site to the CaMn4 cluster in Photosystem II based on solvent accessibility simulations, with implications for substrate water access
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Photochemistry and Molecular Science.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Photochemistry and Molecular Science.
2008 (English)In: Biochimica et Biophysica Acta - Bioenergetics, ISSN 0005-2728, E-ISSN 1879-2650, Vol. 1777, no 2, 140-153 p.Article in journal (Refereed) Published
Abstract [en]

Given the tightly packed environment of Photosystem II (PSII), channels are expected to exist within the protein to allow the movement of small molecules to and from the oxygen evolving centre. In this report, we calculate solvent contact surfaces from the PSII crystal structures to identify such access channels for methanol and water molecules. In a previous study of the effects of methanol on the EPR split S-1-, S-3-, and S-0-signals [Su et al. (2006) Biochemistry 45, 7617-7627], we proposed that methanol binds to one and the same Mn ion in all S-states. We find here that while channels of methanol dimensions were able to make contact with the CaMn4 cluster, only Mn-3 and Mn-4 were accessible to methanol. Combining this observation with spectroscopic data in the literature, we propose that Mn-3 is the ion to which methanol binds. Furthermore, by calculating solvent contact surfaces for water, we found analogous and more extensive water accessible channels within PSII. On the basis of their structure, orientation, and electrostatic properties, we propose functional assignments of these channels as passages for substrate water access to the CaMn4 cluster, and for the exit of O-2 and H+ that are released during water oxidation. Finally, we discuss the possible existence of a gating mechanism for the control of substrate water access to the CaMn4 cluster, based on the observation of a gap within the channel system that is formed by Ca2+ and several mechanistically very significant residues in the vicinity of the cluster.

Place, publisher, year, edition, pages
2008. Vol. 1777, no 2, 140-153 p.
Keyword [en]
photosystem II, channel, solvent contact surface, substrate water access, H+ exit pathway, O-2 exit pathway
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:uu:diva-112434DOI: 10.1016/j.bbabio.2007.08.009ISI: 000253352000004OAI: oai:DiVA.org:uu-112434DiVA: diva2:286195
Available from: 2010-01-14 Created: 2010-01-13 Last updated: 2016-04-22Bibliographically approved

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Ho, Felix M.Styring, Stenbjörn

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