uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
The bacterial toxin ReIE induces specific mRNA cleavage in the A site of the eukaryote ribosome
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
Show others and affiliations
2008 (English)In: RNA: A publication of the RNA Society, ISSN 1355-8382, E-ISSN 1469-9001, Vol. 14, no 2, 233-239 p.Article in journal (Refereed) Published
Abstract [en]

ReIE/ReIB is a well-characterized toxin-anti-toxin pair involved in nutritional stress responses in Bacteria and Archae. ReIE lacks any eukaryote homolog, but we demonstrate here that it efficiently and specifically cleaves mRNA in the A site of the eukaryote ribosome. The cleavage mechanism is similar to that in bacteria, showing the feasibility of A-site cleavage of mRNA for regulatory purposes also in eukaryotes. ReIE cleavage in the A-site codon of a stalled eukaryote ribosome is precise and easily monitored, making "ReIE printing" a useful complement to toeprinting to determine the exact mRNA location on the eukaryote ribosome and to probe the occupancy of its A site.

Place, publisher, year, edition, pages
RNA Society , 2008. Vol. 14, no 2, 233-239 p.
Keyword [en]
bacterial toxin ReIE, eukaryotic ribosomes, mRNA cleavage, initiation complex assembly, toeprinting, ReIE printing
National Category
Biological Sciences
URN: urn:nbn:se:uu:diva-112436DOI: 10.1261/rna.693208ISI: 000252929400006OAI: oai:DiVA.org:uu-112436DiVA: diva2:286198
Available from: 2010-01-14 Created: 2010-01-13 Last updated: 2010-07-19Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Hauryliuk, VasiliEhrenberg, Måns
By organisation
Department of Cell and Molecular Biology
In the same journal
RNA: A publication of the RNA Society
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 146 hits
ReferencesLink to record
Permanent link

Direct link