Printing protein arrays from DNA arrays
2008 (English)In: Nature Methods, ISSN 1548-7091, E-ISSN 1548-7105, Vol. 5, no 2, 175-177 p.Article in journal (Refereed) Published
We describe a method, DNA array to protein array (DAPA), which allows the 'printing' of replicate protein arrays directly from a DNA array template using cell-free protein synthesis. At least 20 copies of a protein array can be obtained from a single DNA array. DAPA eliminates the need for separate protein expression, purification and spotting, and also overcomes the problem of long-term functional storage of surface-bound proteins.
Place, publisher, year, edition, pages
2008. Vol. 5, no 2, 175-177 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-112446DOI: 10.1038/NMETH.1178ISI: 000252750700020PubMedID: 18204456OAI: oai:DiVA.org:uu-112446DiVA: diva2:286210