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The inflammatory response and hyaluronan synthases in the rabbit flexor tendon and tendon sheath following injury
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Hand Surgery.
McCaig Centre for Joint Injury and Arthritis Research, University of Calgary, Calgary, Canada.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Hand Surgery.
2007 (English)In: Journal of Hand Surgery: European Volume, ISSN 1753-1934, Vol. 32, no 5, 581-587 p.Article in journal (Refereed) Published
Abstract [en]

Using a rabbit model of flexor tendon injury, mRNA levels for a subset of relevant molecules involved in inflammatory and fibrotic processes were assessed by reverse transcriptase-polymerase chain reaction 3, 6, 12 and 24 days after injury. Increased levels of COX-2, IL-1beta, MMP-13 and TIMP-1 mRNA were detected in both tendon and tendon sheath following injury, with each molecule exhibiting tissue and time-dependent changes. MMP-13 and TIMP-1 mRNA levels were markedly upregulated in both tissues, whereas COX-2 and IL-1beta predominantly increased in tendon. Both hyaluronan synthase (HAS) 2 and 3 exhibited increases in mRNA levels in tendon tissue after injury, HAS 2 being more pronounced. These findings support the concept that healing in the flexor tendon and the sheath involve different molecular events and that each tissue may require unique modifications if healing is to be enhanced and adhesions reduced.

Place, publisher, year, edition, pages
2007. Vol. 32, no 5, 581-587 p.
Keyword [en]
flexor tendon injury, hyaluronan synthases, interleukin-1B, cyclooxygenase-2, inducible nitric oxide synthase
National Category
Medical and Health Sciences
Research subject
Orthopaedics
Identifiers
URN: urn:nbn:se:uu:diva-119913DOI: 10.1016/j.jhse.2007.05.017ISI: 000251160000021PubMedID: 17950228OAI: oai:DiVA.org:uu-119913DiVA: diva2:301325
Available from: 2010-03-03 Created: 2010-03-03 Last updated: 2011-01-14Bibliographically approved
In thesis
1. Biomolecular Aspects of Flexor Tendon Healing
Open this publication in new window or tab >>Biomolecular Aspects of Flexor Tendon Healing
2010 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Flexor tendon injuries in zone II of the hand (i.e. between the distal volar crease and the distal interphalangeal joint) can be costly for both the afflicted individual and society because of the high cost of a long rehabilitation period, complicated by tendon ruptures or scarring with adhesion formation, causing impaired range of motion. The aim of the present thesis was to characterize more fully the deep flexor tendon, the tendon sheath and their response to injury in a rabbit model in order to find potential targets to improve the outcome of repair.

The intrasynovial rabbit deep flexor tendon differed from the extrasynovial peroneus tendon in the expression of collagens and transforming growth factor-β1 gene expression. Differences were also found in collagen III and proteoglycans between regions of the flexor tendon subjected to either compressive or tensile load.

After laceration and subsequent repair of the flexor tendon, a shift in collagen gene expression from type I to type III occurred. Proteoglycans were generally increased with the notable exception of decorin, a potential inhibitor of the profibrotic transforming growth factor-β1 which was markedly increased during the first two weeks after repair in tendon tissue but remained unaltered in the sheaths. Both vascular endothelial growth factor and basic fibroblast growth factor mRNA levels remained essentially unaltered, whereas insulin-like growth factor-1 increased later in the healing process, suggesting potential beneficial effects of exogenous addition, increasing tendon strength through stimulating tenocyte proliferation and collagen synthesis.

Matrix metalloproteinase-13 mRNA levels increased and remained high in both tendon and sheath, whereas there was only a transient increase of matrix metalloproteinase-3 mRNA in tendon. We could also demonstrate a significant increase of the proportion of myofibroblasts, mast cells and neuropeptide containing nerve fibers in the healing tendon tissue, all components of the profibrotic myofibroblast-mast cell-neuropeptide pathway.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2010. 59 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 1651-6206 ; 544
Keyword
Flexor tendon healing, Growth factor, Metalloproteinase, Collagen, Proteoglycan, Myofibroblast, Hyaluronan synthase, Mast cell
National Category
Surgery
Research subject
Orthopaedics
Identifiers
urn:nbn:se:uu:diva-120304 (URN)978-91-554-7762-2 (ISBN)
Public defence
2010-05-06, Robergsalen, Ing. 40, Akademiska sjukhuset, Uppsala, 13:15 (Swedish)
Opponent
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Biomolecular aspects of flexor tendon healing
Available from: 2010-04-15 Created: 2010-03-11 Last updated: 2010-04-16

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Berglund, Maria

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