A quantitative phospho-proteomic study on rat hippocampal synaptoneurosomes following a single 19-nortestosterone administration.
(English)Manuscript (preprint) (Other academic)
In the past, several studies have been conducted investigating the impact of androgens on the activation of known signaling pathways in cultured primary hippocampal cells and C6 cells. We have recently reported two studies showing altered in vivo phosphorylation of key components in cellular signaling in rat hippocampal synaptoneurosomes after administration of anabolic androgenic steroids. In this paper we report a quantitative liquid chromatography mass spectrometry-based screening for androgen-induced alterations of phosphoproteins in rat hippocampal synaptoneurosomes following a single 19-nortestosterone administration. We identified over 80 phosphorylated peptides with functions associated with the cytoskeleton and transmembrane signaling. DeCyder™ MS based quantitative comparison of the relative phosphopeptide abundances between the two groups of treatment was computed for the majority of the phosphopeptides.
IdentifiersURN: urn:nbn:se:uu:diva-120302OAI: oai:DiVA.org:uu-120302DiVA: diva2:303093