Investigation of Immobilization of Functionalized Magnetic Nanobeads in Rolling CircleAmplified DNA Coils
2010 (English)In: Journal of Physical Chemistry B, ISSN 1520-6106, E-ISSN 1520-5207, Vol. 114, no 10, 3707-3713 p.Article in journal (Refereed) Published
Immobilization characteristics for single-stranded oligonucleotide- functionalized magnetic beads with nominal sizes of 40, 80, 130, and 250 nm in rolling circle amplified (RCA) DNA coils is investigated by employing complex magnetization measurements, dynamic light scattering and fluorescence microscopy. It was found that larger beads in a polydisperse bead size distribution more easily immobilize in the RCA DNA coils than do smaller beads. This may be related to a higher oligonucleotide surface coverage for the larger beads. Furthermore, it was concluded that both bead size and oligonucleotide surface coverage determine whether beads immobilize to give isolated coils with beads or larger clusters of beads and coils. A small bead size and a low oligonucleotide surface coverage favor the first kind of immobilization behavior, whereas a large bead size and a high oligonucleotide surface coverage favor the other. The present findings could be used to optimize both size and surface functionalization of beads employed in substrate-free magnetic biosensors.
Place, publisher, year, edition, pages
2010. Vol. 114, no 10, 3707-3713 p.
Engineering and Technology
Research subject Engineering Science with specialization in Nanotechnology and Functional Materials
IdentifiersURN: urn:nbn:se:uu:diva-120765DOI: 10.1021/jp911251kISI: 000275328400037PubMedID: 20175549OAI: oai:DiVA.org:uu-120765DiVA: diva2:303965