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Quantitative determination of cerebrospinal fluid bilirubin on a high throughput chemistry analyzer
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Chemistry. (biochemical structure and function)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Chemistry. (biochemical structure and function)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Chemistry. (biochemical structure and function)
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2009 (English)In: Clinical laboratory, ISSN 1433-6510, Vol. 55, no 7-8, 283-288 p.Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Subarachnoid hemorrhage is a condition with high rates of mortality and morbidity. The diagnosis requires an urgent cerebral computed tomography scan and also a lumbar puncture if the scan fails to demonstrate intracranial blood. In Sweden the cerebrospinal fluid (CSF) is analyzed by spectrophotometric scanning for the presence of hemoglobin and bilirubin. The aim of the study was to develop a quantitative diazo reagent based analysis of cerebrospinal fluid bilirubin as a replacement for spectrophotometric scanning. METHODS: The CSF bilirubin assay on an Architect C8000 chemistry analyzer was compared with spectrophotometry using patient samples. RESULTS: The method correlates with spectrophotometry, has a good linearity and precision. CONCLUSIONS: Quantitative bilirubin measurement offers shorter turnaround times, simplifies the interpretation of the results and reduces work load in comparison with spectrophotometry.

Place, publisher, year, edition, pages
2009. Vol. 55, no 7-8, 283-288 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-120839ISI: 000271098200003PubMedID: 19894407OAI: oai:DiVA.org:uu-120839DiVA: diva2:304005
Available from: 2010-03-16 Created: 2010-03-16 Last updated: 2011-04-12Bibliographically approved

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