uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Vitrification versus controlled-rate freezing in cryopreservation of human ovarian tissue
Dept of Clinical Science, Technology and Intervention, Div. of Obstetrics and Gynaecology, Karolinska Institutet, Stockholm, Sweden.
Show others and affiliations
2009 (English)In: Human Reproduction, ISSN 0268-1161, E-ISSN 1460-2350, Vol. 24, no 7, 1670-1683 p.Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Controlled-rate freezing of ovarian cortical tissue for preservation of fertility among young women facing chemo- or radio-therapy is a widely accepted procedure. To improve the method for cryopreservation of ovarian tissue, particularly the stroma, we carried out a systematic comparison of vitrification versus slow programmed freezing. METHODS: Ovarian tissue from 20 women, donated during Caesarean section, was used for parallel comparison of survival and detailed light and electron microscopic (EM) morphology of oocytes, granulosa cells and ovarian stroma after freezing (slow freezing and vitrification), thawing and 24-h culture. Using tissue obtained from the same patient, we compared four cryopreservation protocols and fresh tissue. The cryoprotectants used in slow freezing were 1,2-propanediol (PrOH)-sucrose and ethylene glycol (EG)-sucrose. For vitrification, tissues were incubated for 5 or 10 min in three solutions containing a combination of dimethyl sulphoxide (DMSO), PrOH, EG and polyvinylpyrrolidone (PVP). RESULTS: Cryopreservation using controlled-rate freezing and vitrification preserved the morphological characteristics of ovarian tissue generally well. As revealed by morphological analysis, particularly EM, the ovarian stroma was significantly better preserved after vitrification than after slow freezing (P < 0.001). The follicles were similarly preserved after all freezing methods. CONCLUSIONS: Vitrification using a combination of PrOH, EG, DMSO and PVP was comparable to slow freezing in terms of preserving follicles in human ovarian tissue. Ovarian stroma had significantly better morphological integrity after vitrification than after controlled-rate freezing.

Place, publisher, year, edition, pages
2009. Vol. 24, no 7, 1670-1683 p.
Keyword [en]
cryopreservation, human ovarian tissue, ultrastructure, vitrification
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-121039DOI: 10.1093/humrep/dep079ISI: 000267220600020PubMedID: 19359339OAI: oai:DiVA.org:uu-121039DiVA: diva2:304440
Available from: 2010-03-18 Created: 2010-03-18 Last updated: 2010-07-23Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed
By organisation
Department of Women's and Children's Health
In the same journal
Human Reproduction
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 180 hits
ReferencesLink to record
Permanent link

Direct link