EVOLUTION OF HEMATOPOIESIS: AN ASTAKINE INDUCED NOVEL HEMATOPOIETIC FACTOR
(English)Manuscript (preprint) (Other academic)
We report here the cloning and initial characterization of a novel invertebrate hematopoietic factor. This factor was identified from the SSH library with the aim to find the downstream genes of an invertebrate cytokine, astakine 1 in the freshwater crayfish Pacifastacus leniusculus. This Pacifastacus Hematopoietic Factor (PHF) was found to be induced in the primary cell culture of crayfish Hpt cells (precursor of crayfish blood cells) by treatment with astakine 1. Silencing PHF did not affect the renewal of Hpt cells in vitro, but induced the apoptosis rate of Hpt cells. PHF is dominantly present in the blood lineage of crayfish (Hpt cells and blood cells), and in vivo RNAi experiment shows that knockdown of this gene results in severe loss of blood cells in the animal. Our data suggest that crayfish PHF is critical for the survival of not only hemocytes but also the Hpt cells by preventing their apoptosis, thus it plays an important role in the hematopoiesis in crayfish. PHF is a small cysteine rich protein (ca. 9 kDa) with high similarity with the N-terminal region of vertebrate CRIM1 and both of them contains an IGFBP variant motif with unknown function. Our study of PHF may also shed light on the function of this untypical IGFBP motif located in the N-terminal of vertebrate CRIM1.
astakine, hematopoiesis, apoptosis, cytokine, invertebrate, CRIM1
Research subject Biology with specialization in Comparative Physiology
IdentifiersURN: urn:nbn:se:uu:diva-120999OAI: oai:DiVA.org:uu-120999DiVA: diva2:304540