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Dental adhesives with bioactive and on-demand bactericidal properties
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.
2010 (English)In: Dental Materials, ISSN 0109-5641, E-ISSN 1879-0097, Vol. 26, no 5, 491-499 p.Article in journal (Refereed) Published
Abstract [en]

Objectives: The aim of the present work was to perform the first in vitro evaluation of a new interfacial bond-promoting material-and-method concept for on-demand long term bacteria inhibition in dental restoration procedures. Methods: The bioactivity, mechanical bonding strength and photocatalytic bactericidal properties, induced by low dose ultraviolet-A (UV-A) irradiation of dental adhesives containing crystalline titania nanoparticles (NPs), were analyzed. Results: Dental adhesives with a NP content of 20 wt% were shown to be bioactive in terms of spontaneous hydroxylapatite formation upon storage in simulated body fluid and the bioactivity was found to be promoted by chemical etching of the adhesives. The mechanical bonding strength between the adhesives and a HA tooth model was shown to be unaffected by the NPs up to a NP content of 30 wt%. Elimination of Staphylococcus epidermidis in contact with the adhesives was found to depend both on UV photocatalytic irradiation intensity and time. Efficient elimination of the bacteria could be achieved using a UV-A dose of 4.5 J/cm2 which is about 6 times below the safe maximum UV dose according to industry guidelines, and 20 times below the average UV-A dose received during an ordinary sun bed session. Significance: The combined features of bioactivity and on-demand bactericidal effect should open up the potential to create dental adhesives that reduce the incidence of secondary caries and promote closure of gaps forming at the interface towards the tooth via remineralization of adjacent tooth substance, as well as prevention of bacterial infections via on-demand UV-A irradiation.

Place, publisher, year, edition, pages
2010. Vol. 26, no 5, 491-499 p.
Keyword [en]
Antibacterial, Bioactivity, Dental adhesive, Nanoparticles, Photocatalysis, Titanium oxide
National Category
Engineering and Technology
Research subject
Materials Science
URN: urn:nbn:se:uu:diva-121515DOI: 10.1016/j.dental.2010.01.008ISI: 000275815300012PubMedID: 20189237OAI: oai:DiVA.org:uu-121515DiVA: diva2:305545
Available from: 2010-03-24 Created: 2010-03-24 Last updated: 2016-04-19Bibliographically approved
In thesis
1. Titanium Dioxide Photocatalysis in Biomaterials Applications
Open this publication in new window or tab >>Titanium Dioxide Photocatalysis in Biomaterials Applications
2013 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Despite extensive preventative efforts, the problem of controlling infections associated with biomedical materials persists. Bacteria tend to colonize on biocompatible materials and form biofilms; thus, novel biomaterials with antibacterial properties are of great interest. In this thesis, titanium dioxide (TiO2)-associated photocatalysis under ultraviolet (UV) irradiation was investigated as a strategy for developing bioactivity and antibacterial properties on biomaterials. Although much of the work was specifically directed towards dental materials, the results presented are applicable to a wide range of biomaterial applications.

Most of the experimental work in the thesis was based on a resin-TiO2 nanocomposite that was prepared by adding 20 wt% TiO2 nanoparticles to a resin-based polymer material. Tests showed that the addition of the nanoparticles endowed the adhesive material with photocatalytic activity without affecting the functional bonding strength. Subsequent studies indicated a number of additional beneficial properties associated with the nanocomposite that appear promising for biomaterial applications. For example, irradiation with UV light induced bioactivity on the otherwise non-bioactive nanocomposite; this was indicated by hydroxyapatite formation on the surface following soaking in Dulbecco’s phosphate-buffered saline. Under UV irradiation, the resin-TiO2 nanocomposite provided effective antibacterial action against both planktonic and biofilm bacteria. UV irradiation of the nanocomposite also provided a prolonged antibacterial effect that continued after removal of the UV light source. UV treatment also reduced bacterial adhesion to the resin-TiO2 surface.

The mechanisms involved in the antibacterial effects of TiO2 photocatalysis were studied by investigating the specific contributions of the photocatalytic reaction products (the reactive oxygen species) and their disinfection kinetics. Methods of improving the viability analysis of bacteria subjected to photocatalysis were also developed. 

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2013. 57 p.
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 1033
titanium dioxide, photocatalysis, bioactivity, antibacterial effect, metabolic activity assay, biofilm, reactive oxygen species, disinfection kinetics, post-UV
National Category
Nano Technology
urn:nbn:se:uu:diva-160634 (URN)978-91-554-8634-1 (ISBN)
Public defence
2013-05-22, Häggsalen, Ångström laboratory, Lägerhyddsvägen 1, Uppsala, 13:30 (English)
Available from: 2013-04-26 Created: 2011-10-27 Last updated: 2013-08-30Bibliographically approved

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Welch, KenCai, YanlingEngqvist, HåkanStrömme, Maria
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